Spectrometer vs Spectrophotometer

Here is the difference table of Spectrometer vs Spectrophotometer. Both equipment’s are using for chemical analysis. What is a Spectrometer? A spectrometer is an instrument using to measure the intensity of light at different wavelengths within a given spectrum. It is commonly used in scientific research, analytical chemistry, and various industries for a wide range … Read more

Estimation of Sulfanilamide by Colorimetry

Explore the estimation of sulfanilamide, a commonly used antibiotic, through colorimetric analysis in the laboratory. Estimation of Sulfanilamide by Colorimetry In the field of pharmaceutical analysis, accurate and reliable determination of drug concentrations is crucial to ensure product quality and patient safety. One widely used method for quantifying the concentration of drugs is colorimetry. We … Read more

Estimation of Dextrose by Colorimetry

Learn estimation of Dextrose by colorimetry with principle, requirements, reagents, procedure and more. It is useful for biochemical analysis. Estimation of Dextrose by Colorimetry In the field of biochemical analysis, the estimation of dextrose (glucose) plays a vital role in various industries, including food production and clinical diagnostics. Colorimetry, a widely used analytical technique, offers … Read more

How to determine Absorption Maxima?

Learn how to Determine Absorption Maxima definition, procedure. These are key indicators of the electronic transitions occur in molecule. What is Absorption maxima? Absorption maxima are key indicators of the electronic transitions that occur within a molecule. When electromagnetic radiation, typically in the ultraviolet-visible (UV-Vis) region, interacts with a compound. Some certain wavelengths are absorbed … Read more

What is Gel chromatography? principle applications

Learn Gel chromatography principle, applications, advantages and limitations. It also known as size exclusion chromatography (SEC). What is Gel chromatography? Gel chromatography, also known as size exclusion chromatography (SEC) or gel filtration chromatography, is a chromatographic technique used for the separation and purification of biomolecules based on their size. It relies on the differential exclusion … Read more

What is HPTLC? principle and applications

Discover the power of High-Performance Thin Layer Chromatography (HPTLC) in the field of analytical chemistry. What is HPTLC? HPTLC full form is High Performance Thin Layer Chromatography. It is a versatile analytical technique that has revolutionized the field of chemical analysis. It offers a robust and efficient separation and identification platform for various compounds, making … Read more

Principle of HPLC with applications

Learn the principle of HPLC and its various components. It is a powerful analytical tool help to separate and analyze complex mixtures. What is HPLC? High-performance liquid chromatography (HPLC) is a analytical technique using for the separation and analysis of complex mixtures. It is a form of column chromatography in which the sample will dissolve … Read more

What is Affinity chromatography? Principle, theory

Learn What is Affinity chromatography? with its principle, theory, applications, advantages and disadvantages. What is Affinity chromatography? Affinity chromatography is a separation technique using to purify and isolate specific biomolecules from a complex mixture. It is based on the principle of specific interactions between a target molecule and a ligand immobilized on a stationary phase. … Read more

Principle of Paper chromatography

Know about Paper chromatography principle, development techniques, applications, advantages and disadvantages. What is Paper chromatography? Paper chromatography is an analytical technique that is commonly used to separate and identify mixtures of compounds. It involves the use of filter paper as the stationary phase and a liquid solvent as the mobile phase. Principle The principle of … Read more

Thin layer chromatography Principle, Methodology

Learn What is Thin layer chromatography? Principle, Methodology, Rf values, advantages, disadvantages and applications. It can separate and identify small quantities of compounds from a mixture. What is Thin layer chromatography? TLC is a simple and efficient chromatographic technique using for separating and identifying small quantities of compounds from a mixture. It may commonly use … Read more